解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
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神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
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公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::A partial synthesis of the title compound, 4'-(dimethylamino)-17 beta-hydroxy-17 alpha-(1-propynyl)benzo[12,12a]-11 alpha,18-cyclo-12a,12b- dihomo-13 alpha-estr-4-en-3-one 1, is reported. The key step in this synthesis represents an intramolecular alkenylaryl radical cyclization. Treatment of 18-[bromo-5-(dimethylamin...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(94)90026-4
更新日期:1994-03-01 00:00:00
abstract::It has been shown that stress or disease-induced increases in plasma corticosterone result in diminished testosterone secretion from the testes. This article reviews investigations from our laboratories that explore the role of 11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD) in this process. It is proposed that th...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/0039-128x(94)90078-7
更新日期:1994-02-01 00:00:00
abstract::Glycyrrhizic acid (GZA) and glycyrrhetinic acid (GRA) can be determined rapidly and precisely by high-performance liquid chromatography (HPLC) in biological fluids and tissues from experimental animals and humans. From plasma and tissues, GZA and GRA are extracted by organic solvents and the extracts can directly be u...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/0039-128x(94)90088-4
更新日期:1994-02-01 00:00:00
abstract::The 7 alpha-methyl substituent is reported to increase the binding affinity of estradiol for the estrogen receptor (ER). In order to evaluate whether this substituent would improve the in vitro binding characteristics and the in vivo tissue distribution of 18F labeled estrogens that we are developing as positron emiss...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(94)90043-4
更新日期:1994-01-01 00:00:00
abstract::1 alpha, 25-Dihydroxyvitamin D3 (1 alpha, 25-(OH)2D3) has been shown to rapidly increase cytosolic calcium in freshly isolated and cultured rat hepatocytes. The rise in cytosolic calcium is dependent on phospholipase A2 (PLA2) activation and cell alkalinization through the Na+/H+ antiport system. To further characteri...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(93)90008-b
更新日期:1993-10-01 00:00:00
abstract::The LNCaP-Fast Growing Colony (FGC) human prostate cancer cell line proliferates in response to the addition of dihydrotestosterone (DHT) 10(-10)-10(-8) M in charcoal-stripped serum-supplemented media. LNCaP-FGC cells will not attach or proliferate in serum-free conditions. LNCaP-FGC stock cultures were maintained in ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(93)90084-z
更新日期:1993-09-01 00:00:00
abstract::Spectroscopic examination of purified extracts of the rumen content of sheep intoxicated by Brachiaria decumbens revealed the presence of two spirostanes, identified as epi-sarsasapogenin and epi-smilagenin. Sarsasapogenone was obtained by the oxidation of sarsasapogenin. The reduction of sarsasapogenone using lithium...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(93)90043-m
更新日期:1993-08-01 00:00:00
abstract::A direct competitive heterologous enzyme-linked immunosorbent assay (ELISA) was developed and validated to determine follicular fluid estradiol levels of different antral follicular sizes (small, medium, and large) obtained from the ovaries of heifers in the follicular phase of the estrous cycle. Polyclonal antiestrad...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(93)90092-2
更新日期:1993-07-01 00:00:00
abstract::It has now been more than 30 years since the laboratory verification of the localization of estrogen in certain animal tissues. Much has been learned since that time regarding the details of this process, including the presence of specific receptors for these hormones in target tissues, the mechanism of ligand binding...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/0039-128x(93)90069-y
更新日期:1993-06-01 00:00:00
abstract::The effect of a substituent in the 12-position of progestagens was studied. To this end, various approaches toward the preparation of 12 beta-alkyl- and 12-alkylidenenorpregnanes were investigated. Eventually, the desired compounds 17 beta-hydroxy-12 beta-methyl-18a-homo-19-nor-17 alpha-pregn-4-en-20-yn-3-one (37) and...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(92)90020-a
更新日期:1992-11-01 00:00:00
abstract::18,20-Epoxy-11 beta,17 alpha,20 beta,21-tetrahydroxypregn-4-en-3-one was synthesized by the application of hypoiodite reaction to the cortisol acetonide. The intermediary 18-iodo derivative was converted to the 11-oxo steroid by chromic acid prior to silver ion-assisted solvolysis. Removal of the protective group with...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(92)90095-q
更新日期:1992-09-01 00:00:00
abstract::Steroid research at Lederle was initiated in 1946 and continued until about 1971. The areas of research interest involved primarily a study of delta 5,7-steroids, provitamin D3 variants, dehydroepiandrosterone variants, steroidal ketals, corticoids, and steroid conjugates. One of the principal goals was to develop nov...
journal_title:Steroids
pub_type: 历史文章,杂志文章
doi:10.1016/0039-128x(92)90083-l
更新日期:1992-08-01 00:00:00
abstract::The hepatic metabolism of 3-oxoandrost-4-ene-17 beta-carboxylic acid (etienic acid), a probable acidic catabolite of deoxycorticosterone, was investigated using rats prepared with an external biliary fistula. Metabolic products were identified by GC-MS after hydrolysis with beta-glucuronidase and by proton nuclear mag...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(92)90052-b
更新日期:1992-07-01 00:00:00
abstract::Guinea pig adrenal estrogen sulfotransferase from either sex was eluted as a single peak, irrespective of buffer salt concentration, when subjected to fast protein liquid chromatography on gel filtration columns. The same enzyme was consistently eluted in two distinct peaks during chromatofocusing. Adrenal pregnenolon...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(92)90063-f
更新日期:1992-06-01 00:00:00
abstract::Some properties of immunoreactive estradiol secreted by adrenals and ovaries of immature female rats were studied. It was shown that adrenals and ovaries of 15-day-old animals secrete approximately equal amounts of immunoreactive estradiol, with thin-layer chromatogram of immunoreactive material being identical for bo...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(92)90004-s
更新日期:1992-04-01 00:00:00
abstract::In humans, the onset of adrenache has been found to occur with the appearance of the zona reticularis, the inner zone of the adrenal cortex. Since an increase in the volume of adrenal cortex during maturation in the guinea pig has been associated with the growth of the zona reticularis, we were interested in investiga...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(92)90033-6
更新日期:1992-02-01 00:00:00
abstract::The synthesis of a radioiodinated diethylstilbestrol (DES) derivative is described. This derivative was prepared by coupling the previously synthesized active ester of 6-(4-O-diethylstilbestryl)hexanoic acid with mono-[125I]iodotyramine in dry tetrahydrofuran (20 to 22 C, 16 hours). The mono-[125I]iodotyramine was pre...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(92)90092-n
更新日期:1992-01-01 00:00:00
abstract::The uterine milk (UTM) proteins are the major progesterone-regulated proteins secreted by the sheep uterus during pregnancy. Recently, proteins related to the UTM proteins have been identified in uterine secretions of the pregnant cow and sow. The present objective was to determine the time course for induction of the...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90022-n
更新日期:1991-12-01 00:00:00
abstract::To determine the sensitivity of the overnight 1-mg dexamethasone suppression test in diagnosing Cushing's syndrome, we evaluated the cortisol responses of 55 subjects (25 non-obese individuals with body mass index less than 25 kg/m2, 20 obese individuals with body mass index greater than 30 kg/m2, and 10 patients with...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90011-j
更新日期:1991-11-01 00:00:00
abstract::Synthesis of 7-aryl/allyl-substituted androstene derivatives 3a through 3g has been carried out by Grignard reaction on 3 beta,17 beta-diacetoxyandrost-5-en-7-one (2) with aryl/allyl magnesium bromide. Isomeric mixture of products 3b and 3c/3e and 3f/3h was separated by column chromatography. Stereochemical assignment...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90005-g
更新日期:1991-09-01 00:00:00
abstract::A series of steroidal and nonsteroidal Michael acceptors that represent reaction products for 3 alpha-hydroxysteroid dehydrogenase were synthesized and evaluated as potential enzyme-generated inactivators. Introduction of exocyclic olefins either at C-2 or C-6 produced inhibitors with high affinity for the enzyme (0.0...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90030-y
更新日期:1991-08-01 00:00:00
abstract::Sex steroid-binding protein receptor was detected on membranes prepared from human premenopausal endometrium. The binding of sex steroid-binding protein to membranes was specific, saturable, and high affinity. Scatchard analysis showed the presence of two binding sites at different affinities. The addition of estradio...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90058-4
更新日期:1991-06-01 00:00:00
abstract::Key to the production of biologically active steroids is the enzyme 3 beta-hydroxysteroid dehydrogenase-isomerase. Some controversy has arisen concerning the subcellular distribution of this enzyme within steroidogenic cells. The distribution of 3 beta-hydroxysteroid dehydrogenase-isomerase was assessed in subcellular...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90056-2
更新日期:1991-06-01 00:00:00
abstract::Human mammary medullary carcinoma cells (passages 16 to 21) were cultured for 2 days to allow for attachment, followed by 6 days of culture in either fetal calf serum, human cord blood, human amniotic fluid, or growth factors in the presence or absence of purified human alpha-fetoprotein (AFP). When growth factors wer...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90042-t
更新日期:1991-05-01 00:00:00
abstract::Carbon-13 nuclear magnetic resonance spectra for 31 3 beta-hydroxy and acetoxy androstane derivatives bearing vicinal oxygenated functions at ring D with and without oxygenated functions at C-6 are reported. Relative substituent effects are discussed. ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90077-9
更新日期:1991-04-01 00:00:00
abstract::Morphologic changes at the interface of rat endometrial luminal epithelial cells and the stromal cells immediately adjacent were examined and correlated with hypertrophy of the epithelial cells during estradiol (E2) infusion (1 microgram E2/24 h). While the lamina densa in castrate endometrium was thread-like, it beca...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90061-y
更新日期:1991-03-01 00:00:00
abstract::Ventral prostate was used as a system to study the nature and properties of microsomal androgen receptor. The endoplasmic reticulum from rat ventral prostate contains high-affinity, low-capacity binding sites for androgen that are intrinsic to this intracellular compartment. Microsomal androgen receptors are not due t...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90126-g
更新日期:1991-02-01 00:00:00
abstract::17-Hydroxyprogesterone is a well-known precursor of androstenedione in adrenal biosynthesis. This study using sheep adrenal incubations demonstrates that 11-deoxycortisol, the precursor of cortisol synthesis, also can be a precursor of androstenedione. Indeed, our data show that androstenedione synthesis is negatively...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90112-9
更新日期:1991-01-01 00:00:00
abstract::Incubation of aldosterone with confluent layers of A6 (toad kidney) cells leads to its hydroxylation at the 6 beta-position. 6 beta-Hydroxyaldosterone is the major metabolite when the incubation is carried out at pH 6.8, whereas the product comprises 6 beta-hydroxy-17-isoaldosterone accompanied by some 6 beta-hydroxya...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(90)90084-o
更新日期:1990-11-01 00:00:00
abstract::Fast atom bombardment/mass spectrometry or liquid secondary ion mass spectrometry provides the capability for direct analysis of steroid conjugates (sulfates, glucuronides) without prior hydrolysis or derivatization. During the analysis of biologic extracts, limitations on the sensitivity of detection arise from the p...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/0039-128x(90)90014-3
更新日期:1990-10-01 00:00:00
abstract::The synthesis of 13C-labeled steroid hormones is reviewed. Two general approaches are highlighted: partial synthesis in which part of the steroid nucleus is replaced with 13C-labeled synthons, and total synthesis. Examples from both approaches, leading to (3-(3)C)-, (4-(13)C)-, (3,4-(13)C2)-, and (1,2,3,4-(13)C4)- lab...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/0039-128x(90)90012-z
更新日期:1990-10-01 00:00:00
abstract::The concentrations of C-19 steroids were measured in guinea pig and rat adrenals before and after castration as well as after stimulation with adrenocorticotropin hormone (ACTH). Characterization of adrenal C-19 steroids was also carried out by isolation with high-performance liquid chromatography and gas chromatograp...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(90)90060-o
更新日期:1990-08-01 00:00:00
abstract::Ten sex steroids were measured in the peripheral serum and ovarian follicular fluid of female pigs with or without cystic ovarian disease. In general, progestin, especially progesterone, accumulated excessively in the fluid contained in cystic compared with normal follicles. Nonluteinized cystic follicles contained up...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(90)90036-b
更新日期:1990-07-01 00:00:00
abstract::5 alpha-(17R,20R)-14,15-Secocholestane (12) was synthesized from cholesterol (1) in 12 steps. The key intermediate, 5 alpha-cholest-14-en-3 beta-yl acetate (4), underwent ozonization, reduction, hydrolysis, and oxidation to provide 5 alpha-14,15-secocholesta-3,14,15-trione (8). One of the Clemmensen reduction products...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(90)90042-a
更新日期:1990-06-01 00:00:00
abstract::The effect of attachment of a dimethylaminoethoxy or a dimethylaminopropoxy group at the 11 beta-position of estradiol (E2) on its relative binding affinity (RBA) to estrogen receptor (ER) and intrinsic biologic activity is described. The binding of 11 beta-[2-(N,N-dimethylamino) ethoxy]estra-1,3,5(10)-triene-3,17 bet...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(90)90022-4
更新日期:1990-05-01 00:00:00
abstract::The ring A monoglucuronides and monosulfates of 2-hydroxyestriol were synthesized from 2-hydroxyestriol 16,17-diacetate by means of the Koenigs-Knorr reaction with methyl alpha-acetobromoglucuronate and sulfation with sulfur trioxide-pyridine complex, respectively. The conjugated positions of these compounds were defi...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(90)90009-z
更新日期:1990-03-01 00:00:00
abstract::Reaction kinetics of the aromatase enzyme and of a new nonsteroidal aromatase inhibitor, R 76 713 (6-[(4-chlorophenyl)(1H-1,2,4-triazol-1-yl)-methyl]-1-methyl-1H- benzotriazole), were studied in ovarian homogenates obtained from pregnant mare's serum gonadotropin (PMSG)-injected female Wistar rats. The Km (Michaelis c...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(90)90027-9
更新日期:1990-02-01 00:00:00
abstract::The steroid receptor-positive human ovarian cancer (BG-1) was evaluated to determine its usefulness as a tumor model. This tumor grows in intact male and female nude mice without hormone supplements. Moreover, its growth was significantly accelerated in ovariectomized mice, and the increased growth rate could be rever...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(89)90083-4
更新日期:1989-12-01 00:00:00
abstract::Studies were carried out on the effect of oxygen tension on progesterone (P) accumulation in rat granulosa cell cultures. At 1-2% oxygen, basal, luteinizing hormone (LH)-stimulated, and follicle stimulating hormone (FSH)-stimulated P accumulations were 20, 18, and 11%, respectively, of P levels at 20% oxygen. Basal P ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(89)90047-0
更新日期:1989-11-01 00:00:00
abstract::In the present study the possible dual effects of adenosine as substrate and adenosine receptor agonist in rat granulosa cells, cumulus-oocyte complexes, luteal cells and ovarian membranes are discussed. Adenosine is an indispensable compound in cell energy metabolism, as precursor to cofactors, second messenger and n...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(89)90045-7
更新日期:1989-11-01 00:00:00